New PDF release: Chemically Modified Electrodes (Advances in Electrochemical

By Richard C. Alkire, Dieter M. Kolb, Jacek Lipkowski, Phil N. Ross

ISBN-10: 3527314202

ISBN-13: 9783527314201

With contributions from a world team of professional authors, this ebook comprises the newest tendencies in tailoring interfacial houses electrochemically. The chapters hide numerous natural and inorganic compounds, with purposes starting from electrochemistry to nanotechnology and biology.
Of curiosity to actual, floor and electrochemists, fabrics scientists and physicists.

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Extra resources for Chemically Modified Electrodes (Advances in Electrochemical Sciences and Engineering)

Sample text

In this sensor, one end of the molecular wire is attached to ferrocene dimethylamine with a covalent link formed between one of the amine groups on the ferrocene and the carboxyl group on the wire. To the other amine is attached the antibody-binding epitope for the antibody, in this proof-of-concept study the epitope is biotin. Electron transfer can be readily achieved to the ferrocene molecule but upon antibody binding to this interface, the electrochemical signal is dramatically reduced. The immunosensor based on the modulation of electrochemical signals by protein binding can be used in two modes.

Changing the ratio of the two components in solution was shown to be able to influence the amount of glucose oxidase interrogated electrochemically at the electrode surface. The optimal ratio in the assembly solution was 1 : 30 molecular wire:carboxy phenyl derivative. 4 pmol cmÀ2. 0 nm [85]. Increasing the ratio of molecular wires results in a decrease in surface coverage that is attributed to the surface density of molecular wires crowding the interface such that there is insufficient spacing between wires to allow all the enzymes attached to the surface to fit over a wire and at lower ratios of molecular wires there are insufficient number of wires to get a complete monolayer of enzyme immobilized.

Such enhancement of electron transfer has facilitated the development of biosensors for direct quantification of the redox-active biomolecule, with greater sensitivity, wider detection limits and decreased response times. The effective quantification of biological molecules using nanostructured electrodes requires that nanostructures attached to electrodes are able to electrochemically catalyze redox reactions of the biological molecules. Electrodes functionalized for this purpose can be broadly categorized into metallic-nanoparticle and carbon-nanotube structures.

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Chemically Modified Electrodes (Advances in Electrochemical Sciences and Engineering) by Richard C. Alkire, Dieter M. Kolb, Jacek Lipkowski, Phil N. Ross


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